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AssigneeGE Healthcare Bio-Sciences AB
InventorsBo-Lennart Johansson, Hans J. Johansson, Anders Ljunglöf, Jean-Luc Maloisel, Nicolas Thevenin
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The present invention relates to a method of separating antibodies from contaminants in a solution, which method comprises contacting the solution with a chromatography resin comprised of a support to which multi-modal ligands have been immobilised, wherein a multi-modal ligand comprises at least one cation-exchanging group and at least one aromatic or heteroaromatic ring system. In one embodiment, the ring-forming atoms of the aromatic or hereoaromatic entity are selected among C, S or O, and the cation exchanging group is a weak cation exchanger. The present method may be used as a single step procedure or as a polishing step following a capture on a Protein A column.
A method of separating antibodies from one or more contaminants in a solution, comprising contacting the solution, which is an antibody-containing eluate from an affinity chromatography resin, with a second chromatography resin comprised of a support to which multi-modal ligands have been immobilised, wherein a multi-modal ligand comprises at least one cation-exchanging group and at least one aromatic or heteroaromatic ring system, to adsorb antibodies and/or contaminants to the resin.
Dependencies are inferred from the claim text.