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AssigneeWyeth
InventorsRobert John Zagursky, Amy Wadhams Masi, Bruce Arthur Green, Deb Narayan Chakravarti, David Parrish Russell, Joseph Lawrence Wooters
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The present invention relates to newly identified open reading frames comprised within the genomic nucleotide sequence of Streptococcus pneumoniae, wherein the open reading frames encode polypeptides that are surface localized on Streptococcus pneumoniae. Thus, the invention relates to Streptococcus pneumoniae open reading frames that encode polypeptide antigens, polypeptides, preferably antigenic polypeptides, encoded by the Streptococcus pneumoniae open reading frames, vectors comprising open reading frame sequences and cells or animals transformed with these vectors. The invention relates also to methods of detecting these nucleic acids or polypeptides and kits for diagnosing Streptococcus pneumoniae infection. The invention finally relates to pharmaceutical compositions, in particular immunogenic compositions, for the prevention and/or treatment of bacterial infection, in particular infections with Streptococcus pneumoniae. In particular embodiments, the immunogenic compositions are used for the treatment or prevention of systemic diseases which are induced or exacerbated by Streptococcus pneumoniae. In other embodiments, the immunogenic compositions are used for the treatment or prevention of non-systemic diseases, particularly of the otitis media, which are induced or exacerbated by Streptococcus pneumoniae.
An isolated Streptococcus pneumoniae polynucleotide comprising at least 95% identity to the full length nucleotide sequence of SEQ ID NO:91, and wherein the polynucleotide encodes an immunogenic polypeptide.
An isolated polynucleotide, wherein the polynucleotide is a complement to the nucleotide sequence of SEQ ID NO:91.
An isolated polynucleotide which hybridizes to the full length nucleotide sequence of SEQ ID NO:91, or a complement thereof, under high stringency hybridization conditions, wherein the high stringency hybridization is at 65° C. with lxSSC followed by a 65° C. wash with O.3×SSC, and wherein the polynucleotide encodes an immunogenic polypeptide.
An isolated Streptococcus pneumoniae polynucleotide, wherein the polynucleotide comprises the nucleotide sequence of SEQ ID NO:91, and encodes a polypeptide, selected from the group consisting of:
An isolated recombinant expression vector comprising a polynucleotide having at least 95% identity to the full length nucleotide sequence of SEQ ID NO:91, wherein the polynucleotide encodes for an immunogenic polypeptide.
An isolated polynucleotide of a Streptococcus pneumoniae, wherein the polynucleotide encodes a polypeptide comprising the amino acid sequence of SEQ ID NO:306.
An isolated polynucleotide comprising the nucleotide sequence of SEQ ID NO:
An isolated polynucleotide that encodes a polypeptide comprising the amino acid sequence of SEQ ID NO:
An isolated polynucleotide that encodes a polypeptide comprising an amino acid sequence having at least 95% identity to the amino acid sequence of SEQ ID NO:
An immunogenic composition comprising a polynucleotide having a nucleotide sequence of SEQ ID NO:91 and is comprised in an expression vector.
Dependencies are inferred from the claim text.