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AssigneeMonsanto Technology LLC
InventorsMurtaza F. Alibhai, James D. Astwood, Charles A. McWherter, Hugh A. Sampson
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Modified proteins are disclosed that maintain enzymatic and insecticidal activity while displaying reduced or eliminated allergenicity. Epitopes which bind to anti-patatin antibodies were identified, and removed via site directed mutagenesis. Tyrosines were observed to generally contribute to the allergenic properties of patatin proteins. Removal of glycosylation sites was observed to reduce or eliminate antibody binding. Permuteins are also disclosed which have a rearranged amino acid sequence while retaining enzymatic activity. Deallergenized proteins and permuteins can be used as insecticidal materials, as nutritional supplements, and as immunotherapeutic agents.
An isolated nucleic acid molecule segment comprising a structural nucleic acid sequence, said structural nucleic acid sequence encoding a permutein protein comprising (amino acids 269-386 of SEQ ID NO:2)-linker-(amino acids 24-268 of SEQ ID NO:2), wherein said permutein protein is modified by replacing one or more amino acids in positions 264-277 of SEQ ID NO:2 with alanine, glutamic acid, phenylalanine, proline, serine or glutamine and displays reduced binding to anti-acyl lipid hydrolase antibodies with respect to the binding of unmodified acyl lipid hydrolase protein to the anti-acyl lipid hydrolase antibodies.
An isolated nucleic acid molecule segment comprising a structural nucleic acid sequence, said structural nucleic acid sequence encoding a permutein protein comprising (amino acids 269-386 of SEQ ID NO:2)-linker-(amino acids 24-268 of SEQ ID NO:2) and exhibiting corn rootworm insecticidal activity and acyl lipid hydrolase activity.
Dependencies are inferred from the claim text.