Antibodies specific for sclerostin and methods of screening and use therefor
AssigneeCelltech R&D Limited
InventorsDavid G. Winkler, Jiye Shi, John Latham
Life of this patent
Estimating the term…
Abstract
Compositions and methods relating to antibodies that specifically bind to TGF-beta binding proteins are provided. These methods and compositions relate to altering bone mineral density by interfering with the interaction between a TGF-beta binding protein sclerostin and a TGF-beta superfamily member, particularly a bone morphogenic protein. Increasing bone mineral density has uses in diseases and conditions in which low bone mineral density typifies the condition, such as osteopenia, osteoporosis, and bone fractures.
Claims16
An isolated antibody, or an antigen-binding fragment thereof, that binds to an epitope within residues 86-111 of a SOST polypeptide set out in SEQ ID NO:
- 1, and that is produced by immunizing a non-human animal with a peptide of no more than 75 amino acids of SEQ ID NO:
- 1.
A method for identifying an antibody that increases bone mineral content comprising:
- (a) contacting (i) an antibody that specifically binds to a SOST polypeptide set out in SEQ ID NO:
- 1 with (ii) at least one SOST peptide of no more than 75 amino acids of SEQ ID NO:
- 1 comprising an amino acid sequence set out in SEQ ID NO:
- 53 under conditions and for a time sufficient to permit formation of an antibody/SOST peptide complex;
- and (b) detecting a level of antibody/SOST peptide complex, and thereby detecting the presence of an antibody that increases bone mineral content.
An antibody, or an antigen-binding fragment thereof, that binds to an epitope within residues 86-111 of a SOST polypeptide set out in SEQ ID NO:
- 1, and that is produced by immunizing a non-human animal with a peptide of no more than 50 consecutive amino acids of SEQ IID NO:
- 1.
Dependencies are inferred from the claim text.