Recombinant mycobacteria overexpressing D-alanine ligase gene and uses therefore
AssigneeBoard of Regents, University of Nebraska-Lincoln
InventorsRaul G. Barletta, Zhengyu Feng
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Abstract
Recombinant mycobacterial strains which overproduce essential biosynthetic enzymes of pathogenic mycobateria are provided. These strains overproduce enzymes involved in the synthesis and incorporation of D-alanine into mycobacterial peptidoglycan, the backbone of the mycobacterial cell wall. These overproducing strains may be used as reference strains in in vitro screening methods to identify antimycobacterial agents.
Claims10
A recombinant plasmid pBUN276 on deposit with the American Type Culture Collection as accession number PTA-8190.
A recombinant plasmid, comprising at least one genomic DNA fragment encoding M. tuberculosis Ddl, said at least one DNA fragment fused in frame with the first six codons of M. bovis BCG hsp60 gene, and operably linked to the promoter of the M. bovis BCG hsp60 gene, wherein said at least one DNA fragment is cloned into an E. coli-mycobacterium shuttle vector.
Dependencies are inferred from the claim text.