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US 7,371,542 B2Granted
Gene expression system
AssigneeCytos Biotechnology AG
InventorsLidia Ivanova, Philippe Saudan
Life of this patent
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Abstract
The present invention relates to nucleic acid molecules and in particular to vectors, comprising at least one gene of interest, at least one scaffold/matrix attached region (S/MAR), at least one origin of replication, and at least one replication initiation factor, cells comprising these, processes for their propagation, and their use, in particular for the high level expression of proteins which can be used as medicaments.
Claims98
A nucleic acid molecule comprising:
- (a) at least one gene of interest;
- (b) at least one scaffold/matrix attached region (S/MAR), wherein said at least one S/MAR originates from the 5′ region of the interferon beta gene (SEQ ID NO:
- 3);
- (c) at least one origin of replication (ORI);
- and
- (d) at least one nucleic acid sequence encoding a replication initiation factor capable of recognizing said at least one origin of replication;
- wherein the first of said at least one origin of replication is OriP, and wherein the first of said at least one nucleic acid sequence encoding a replication initiation factor comprises the coding sequence for the EBNA-1 protein.
4 more elements
An expression system comprising
- (a) at least one gene of interest;
- (b) at least one scaffold/matrix attached region (S/MAR), wherein said at least one S/MAR originates from the 5′ region of the interferon beta gene (SEQ ID NO:
- 3);
- (c) at least one origin of replication (ORI);
- and
- (d) at least one nucleic acid sequence encoding a replication initiation factor capable of recognizing said at least one origin of replication;
- wherein the first of said at least one origin of replication is OriP, and wherein the first of said at least one nucleic acid sequence encoding a replication initiation factor comprises the coding sequence for the EBNA-1 protein.
4 more elements
A recombinant host cell comprising
- (a) at least one gene of interest;
- (b) at least one scaffold/matrix attached region (S/MAR), wherein said at least one S/MAR originates from the 5′ region of the interferon beta gene (SEQ ID NO:
- 3);
- (c) at least one origin of replication (ORI);
- and
- (d) at least one nucleic acid sequence encoding a replication initiation factor capable of recognizing said at least one origin of replication;
- wherein the first of said at least one origin of replication is OriP, and wherein the first of said at least one nucleic acid sequence encoding a replication initiation factor comprises the coding sequence for the EBNA-1 protein.
4 more elements
Dependencies are inferred from the claim text.