Rescue of mumps virus from cDNA
AssigneeWyeth
InventorsDavid K. Clarke, J. Erik Johnson, Mohinderjit S. Sidhu, Stephen A. Udem
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Abstract
This invention relates to a method for recombinantly producing, via rescue of mumps virus, a nonsegmented, negative-sense, single-stranded RNA virus, and immunogenic compositions formed therefrom. Additional embodiments relate to methods of producing the mumps virus as an attenuated and/or infectious virus. The recombinant viruses are prepared from cDNA clones, and, accordingly, viruses having defined changes, including nucleotide/polynucleotide deletions, insertions, substitutions and re-arrangements, in the place of the genome are obtained.
Claims1
A method for producing a recombinant mumps virus comprising;
- in at least one host cell, conducting transfection or transformation, in media, of a rescue composition which comprises (i) a transcription vector comprising an isolated nucleic acid molecule which comprises a polynucleotide sequence encoding an antigenome of mumps virus, or variant polynucleotide sequence thereof, and (ii) at least one expression vector which comprises one more isolated nucleic acid molecule(s) comprising a polynucleotide sequence encoding the trans-acting proteins (NP, P and L) necessary for encapsidation, transcription and replication;
- under conditions sufficient to permit the co-expression of said vectors and the production of the recombinant virus;
- wherein the isolated nucleic acid molecule encoding an antigenome of mumps virus comprises the polynucleotide sequence selected from the group consisting of SEQ. ID NOS. 1, 11 and 12;
- and wherein the recombinant virus is harvested.
Dependencies are inferred from the claim text.