Self-assembling recombinant papillomavirus capsid proteins
AssigneesThe United States of America as represented by the Department of Health and Human Services, Deutsches Krebsforschungszentrum, The German Cancer Research Center
InventorsDouglas R. Lowy, John T. Schiller, Reinhard Kirnbauer
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Abstract
Recombinant papillomavirus capsid proteins that are capable of self assembly into capsomer structures and viral capsids that comprise conformational antigenic epitopes are provided. The capsomer structures and viral capsids, consisting of the capsid proteins that are expression products of a bovine, monkey or human papillomavirus L1 conformational coding sequence proteins, can be prepared as vaccines to induce a high titer neutralizing antibody response in vertebrate animals. The self assembling capsid proteins can also be used as elements of diagnostic immunoassay procedures for papillomavirus infection.
Claims23
An isolated or purified genetic construct comprising a HPV16 L1 open reading frame (ORF) encoding a HPV 16 L1 having Aspartate at amino acid 202 instead of Histidine and a vector wherein the ORF is under the control of a promoter of the vector.
An isolated or purified genetic construct comprising a HPV16 L1 open reading frame (ORF) encoding a HPV16 L1 having Aspartate at amino acid 202 instead of Histidine and a vector wherein the ORF is under the control of a promoter of the vector, and further comprising a HPV16 L2 open reading frame (ORF) encoding a HPV16 L2.
An isolated or purified genetic construct comprising a papillomavirus open reading frame (ORF) encoding a capsid protein wherein said papillomavirus ORF consists essentially of a HPV16 L1 ORF encoding a HPV16 L1 having Aspartate at amino acid 202 instead of Histidine, and a vector, wherein the ORF is under the control of a promoter of the vector.
Dependencies are inferred from the claim text.