Back to results
US 7,358,069 B2Granted
Vector constructs
AssigneeDevgen NV
InventorsGeert Plaetinck, Jean-pierre Renard, Thierry Bogaert
Life of this patent
Estimating the term…
Abstract
Improved vector constructs useful in the expression of double-stranded RNA are provided. The constructs are particularly useful for expression of double-stranded RNA in vitro and in vivo.
Claims28
A DNA construct that produces double stranded RNA comprising:
- a) a first promoter and
- b) a second promoter,
- in which the first and second promoter are in opposite orientation to each other and define:
- c) an inter-promoter region positioned downstream of the 3′ end of the first promoter and downstream of the 3′ end of the second promoter;
- and which DNA construct further comprises:
- d) at least one cloning site positioned in the inter-promoter region;
- and
- e) a first transcription terminator, positioned, as seen from the 3′ end of the first promoter, downstream of the first promoter and downstream of the at least one cloning site, wherein the first transcription terminator is operably linked to the first promoter.
5 more elements
Dependencies are inferred from the claim text.