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US 7,351,957 B2Granted
Broad ion fragmentation coverage in mass spectrometry by varying the collision energy
AssigneeMDS Inc.
InventorsNic Bloomfield, Yves Leblanc
Life of this patent
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Abstract
In the field of mass spectrometry, a method of obtaining a mass spectrum enriched with fragment ions while retaining the precursor ion. The technique includes varying the collision energy experienced by the precursor ion such that a range of fragmentations occur. Related methods are also disclosed for obtaining MS, MS2, MS3 and MSn spectra which are enriched with fragment ions.
Claims27
A method of fragmenting ions, comprising:
- generating a stream of ions;
- injecting said stream into a collision cell over a period of time, to thereby promote collision induced dissociation;
- and
- varying the collision energy experienced by said stream during injection into said cell.
1 more element
Apparatus for fragmenting ions, comprising:
- means for generating a stream of ions;
- means for injecting said stream into a collision cell over a period of time, to thereby promote collision-induced dissociation of said ions;
- and
- means for varying the collision energy experienced by said stream during injection into said cell.
1 more element
A mass spectrometer, comprising:
- first and second quadrupole rod sets arranged in linear formation, the first rod set being controlled to isolate selected precursor ions, the second rod set being enclosed in a collision chamber having a background gas pressure significantly higher than the ambient environment of the first rod set;
- an ionization device for ionizing a substance and injecting a stream of ions into the first rod set;
- means for varying the voltage applied to the ion stream as it is injected into the collision cell so as to vary the collision energy experienced by said ions over a pre-determined energy range;
- and
- mass filter means for obtaining a mass spectrum from ions emanating from the second rod set.
2 more elements
A triple quadrupole mass spectrometer, comprising:
- first, second and third quadrupole rod sets arranged in linear formation, the first rod set being controlled to isolate selected precursor ions, the second rod set being enclosed in a collision chamber having a background gas pressure significantly higher than the ambient environment of the first and third rod sets, and the third rod set being controlled as a linear ion trap;
- an ionization device for ionizing a substance and injecting a stream of ions into the first rod set;
- and
- means for varying the voltage applied to the ion stream as it is injected into the collision cell so as to vary the collision energy experienced by said ions over a pre-determined energy range.
1 more element
Dependencies are inferred from the claim text.