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US 7,351,876 B2Granted
Efficient nuclear transfer with primordial gametes
AssigneeAgrobiogen Gmbh Biotechnologie
InventorsGottfried Brem, Gabriela Durcova-Hills, Sigrid Müller, Wolfgang Schernthaner, Hendrik Wenigerkind, Eckhard Wolf, Valeri Zakhartchenko
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Abstract
This invention relates to a method for breeding animals via cloning and the animals obtainable by the method, in particular a method for reproducing animal embryos via efficient nuclear transfer with primordial gametes.
Claims4
A method of producing a bovine blastocyst, the method comprising the steps of:
- (a) isolating a bovine primordial gamete from a bovine foetus by mechanically selecting the bovine primordial gamete from cells obtained from the bovine foetus;
- (b) combining directly the nucleus of the bovine primordial gamete with an enucleated bovine oocyte to form a cell;
- (c) activating the cell obtained in step (b);
- and
- (d) cultivating the cell in an in vitro system for a time that is sufficient to form the bovine blastocyst.
2 more elements
Dependencies are inferred from the claim text.