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US 7,351,819 B2Granted
Method of detecting norwalk-like virus (GII)
AssigneeBML, Inc.
InventorsTsutomu Kageyama, Shigeyuki Kojima, Shuetsu Fukushi, Fuminori Hoshino, Kazuhiko Katayama
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Abstract
A method of detecting a virus in a specimen, whereby a Norwalk-like virus (GII) is detected by using as an index the nucleic acids of a complementary nucleotide sequence corresponding to the 4851- to 5450-positions of the nucleotide sequence of the cDNA of the prototype (standard strain) of the Norwalk-like virus (GII); and a detection kit for performing this method.
Claims12
A method of detecting a Norwalk-like virus (GII) in a sample comprising:
- (a) performing a nucleic acid amplification reaction on a sample using at least two oligonucleotide primers, wherein a first oligonucleotide primer comprises a nucleotide sequence of at least 10 consecutive nucleotides of a region consisting of nucleotides 138 to 178 of the polynucleotide sequence set forth in SEQ ID NO:14 and a second oligonucleotide primer comprises a nucleotide sequence complementary to at least 10 consecutive nucleotides of a region consisting of nucleotides 230 to 257 of the polynucleotide sequence set forth in SEQ ID NO:14, and
- (b) detecting an amplification product, thereby detecting a Norwalk-like virus (GII) in a sample.
A method of detecting a Norwalk-like virus (GII) in a sample comprising:
- (a) performing a nucleic acid amplification reaction on a sample to obtain an amplification product, said amplification product comprising a polynucleotide sequence complementary to positions 192 to 217 of SEQ ID NO:14, and
- (b) detecting the amplification product using an oligonucleotide probe, said oligonucleotide probe comprising a polynucleotide sequence complementary to the amplification product.
Dependencies are inferred from the claim text.