Method, use and kit for separating albumin from contaminants in a liquid
AssigneeGE Healthcare Bio-Sciences AB
InventorMakonnen Belew
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Abstract
The present invention is a method of separating recombinant human serum albumin (rHSA) from low molecular weight contaminants in a liquid, which method comprises the steps of: (a) providing a separation medium, which includes anion-exchanging ligands coupled to a base matrix; and (b) contacting the liquid with the separation medium to adsorb the rHSA to the ligands. In one embodiment, the functional groups of the ligands are weak anion-exchanging groups, preferably secondary amines, and the density of ligands on the base matrix is relatively high.
Claims7
A method of separating recombinant human serum albumin (rHSA) from low molecular weight rHSA degradation products in a liquid, which method comprises the step of
- (a) providing a weak anion-exchange separation medium on a base matrix suitable for separating rHSA from said low molecular weight rHSA degradation products;
- (b) contacting the liquid containing said rHSA and low molecular weight rHSA degradation products with the separation medium under conditions that allow the rHSA to adsorb and the degradation products to wash through;
- and
- (c) eluting said rHSA from the separation medium using a salt gradient that releases said rHSA from the ligands.
Dependencies are inferred from the claim text.