Surface modification of metals for biomolecule detection using surface enhanced Raman scattering (SERS)
AssigneeIntel Corporation
InventorsTae-Woong Koo, Selena Chan, Xing Su, Jingwu Zhang, Lei Sun
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Abstract
The present invention is based on the discovery that the methods described herein for the production of metallic colloids result in colloids exhibiting increased signal enhancement and reproducibility for the SERS detection of biomolecules. Thus, using the methods of the invention, a wide variety of biomolecules can be detected with a greater sensitivity and reliability.
Claims22
A method for producing a cluster of surface-modified metallic colloid comprising (1) metallic colloid comprising a metal and (2) a Raman-enhancing organic molecule on a surface of the metallic colloid, the method comprising:
- preparing a solution comprising cations of the metal and a reducing agent by dissolving the cations and the reducing agent in the solution, subsequently heating the solution to produce the metallic colloid, modifying the metallic colloid by attaching the Raman enhancing organic molecule to the surface of the metallic colloid to produce the surface modified metallic colloid, wherein the Raman-enhancing organic molecule comprises a moiety that has an affinity for the metallic colloid and another moiety that has an affinity for a biomolecule, and aggregating a plurality of the surface-modified metallic colloid to form the cluster of surface-modified metallic colloid.
A method for producing a cluster of surface-modified metallic colloid comprising (1) metallic colloid comprising a metal and (2) a Raman-enhancing organic molecule on a surface of the metallic colloid, the method comprising:
- preparing a solution comprising cations of the metal and a reducing agent by dissolving the cations and the reducing agent in the solution, subsequently heating the solution to produce the metallic colloid, modifying the metallic colloid by attaching the Raman-enhancing organic molecule to the surface of the metallic colloid to produce the surface-modified metallic colloid, wherein the Raman-enhancing organic molecule comprises a moiety that has an affinity for the metallic colloid and another moiety that has an affinity for a biomolecule, and
- aggregating a plurality of the surface-modified metallic colloid to form the cluster of surface-modified metallic colloid. wherein the cluster has a size ranging from about 50 nm to 200 nm.
Dependencies are inferred from the claim text.