Products and methods for single parameter and multiparameter phenotyping of cells
OwnerDan A. Pankowskyinventor
Life of this patent
Estimating the term…
Abstract
A method of single parameter and multiparameter characterizing of cells, particularly immunophenotyping of cells, is provided. The method preferably uses antibody coated microspheres which are adapted to bind to specific types of cells. One or more sets of coated microspheres are added simultaneously or sequentially to a suspension of cells and bind the cells they are adapted to bind. Cells may bind to one or more microspheres. The suspension is then filtered to trap bead-cell complexes. The complexes are preferably stained and then examined to characterize the cells, preferably the cells bound to the microspheres. A kit and apparatus for performing the method are also provided.
Claims23
A method of processing cells comprising the steps of:
- a) providing a suspension of cells in a liquid medium,
- b) contacting a group of beads with said suspension, each of said beads being coated with a binding substance or being magnetic such that each bead is specifically adapted to bind to at least one of said cells,
- c) incubating said beads with said suspension for a period of time effective to permit said cells to bind to said beads to form bead-cell complexes, each bead-cell complex comprising a bead and a cell,
- d) separating said bead-cell complexes from said suspension by filtration through a filter, wherein said filter permits effective separation of said bead-cell complexes from said suspension, and transferring to a glass slide said bead-cell complexes separated by said filtration, and
- e) examining said separated bead-cell complexes and characterizing said cells.
Dependencies are inferred from the claim text.