Use of a transgene encoding a vertebrate phytase to increase capacity to utilize phytic acid in livestock feed
AssigneesNorth Carolina State University, The United States of America as represented by the Department of Health and Human Services, NIH, University of Rochester
InventorsStephen Shears, Paul R. Reynolds, James N. Petitte
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Abstract
The present invention provides an isolated animal cell comprising an exogenous nucleic acid encoding a mutated phytase, wherein the cell produces phytase and secretes the phytase from the cell. The present invention also provides an animal having a phenotype not naturally occurring, characterized by secretion of phytase into the lumen of the gastrointestinal tract of the animal, the phenotype being conferred by a transgene contained in the cells of the animal, the transgene comprising a nucleic acid sequence encoding phytase and methods of making said animal.
Claims8
An isolated animal cell comprising an exogenous nucleic acid encoding phytase, wherein the phytase comprises a mutation, further wherein the mutation comprises a deletion of the C-terminal tetrapeptide, and the nucleic acid encoding phytase is functionally expressed in the cell and the phytase is secreted from the cell.
An isolated mammalian cell comprising an exogenous nucleic acid encoding phytase, wherein the phytase is a mutated phytase comprising a deletion of the C-terminal tetrapeptide, and further wherein the cell expresses the nucleic acid encoding phytase and secretes phytase from the cell.
An isolated animal cell comprising an exogenous nucleic acid encoding phytase, wherein the phytase is a mutated multiple inositol polyphosphate phosphatase comprising a deletion of the C-terminal tetrapeptide, and further wherein the cell expresses the nucleic acid encoding phytase and secretes phytase from the cell.
Dependencies are inferred from the claim text.