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US 7,351,569 B2Granted
Aldolase and production process of substituted α-keto acids
AssigneeAjinomoto Co., Inc.
InventorsMasakazu Sugiyama, Kunihiko Watanabe
Life of this patent
Estimating the term…
Abstract
4-(Indol-3-ylmethyl)-4-hydroxy-2-oxoglutarate, which is useful as an intermediate in the synthesis of monatin, may be synthesized from indole pyruvic acid and pyruvic acid (and/or oxaloacetic acid) by using a novel aldolase derived from the genus Pseudomonas, Erwinia, Flavobacterium, or Xanthomonas.
Claims15
An isolated polynucleotide of following (a) or (b):
- (a) a polynucleotide having the nucleotide sequence of SEQ ID NO:
- 1 or the nucleotide sequence of nucleotides 444 to 1118 of SEQ ID NO:
- 1 or nucleotides 456 to 1118 of SEQ ID NO:
- 1;
- (b) a polynucleotide that hybridizes under stringent conditions with a polynucleotide having the nucleotide sequence complementary to the full-length nucleotide sequence of SEQ ID NO:
- 1 or the nucleotide sequence of nucleotides 444 to 1118 of SEQ ID NO:
- 1 or nucleotides 456 to 1118 of SEQ ID NO:
- 1, and encodes a protein having aldolase activity, wherein said stringent conditions comprise a wash step in 0.1×SSC and 0.1% SDS at 65° C.
5 more elements
An isolated polynucleotide of following (c) or (d):
- (c) a polynucleotide that encodes a protein comprising the amino acid sequence of SEQ ID NO:
- 2 or the amino acid sequence of residue numbers 5 to 225 of SEQ ID NO:
- 2;
- (d) a polynucleotide that encodes a protein having an amino acid sequence that contains a substitution, deletion, insertion, or addition of one to ten amino acid residues in the amino acid sequence of SEQ ID NO:
- 2 or the amino acid sequence of residue numbers 5 to 225 of SEQ ID NO:
- 2, and has aldolase activity.
3 more elements
A recombinant DNA produced by ligating into a vector DNA a polynucleotide selected from the group consisting of:
- (a) a polynucleotide having the nucleotide sequence of SEQ ID NO:
- 1 or the nucleotide sequence of nucleotides 444 to 1118 of SEQ ID NO:
- 1 or nucleotides 456 to 1118 of SEQ ID NO:
- 1;
- (b) a polynucleotide that hybridizes under stringent conditions with a polynucleotide having the nucleotide sequence complementary to the full-length nucleotide sequence of SEQ ID NO:
- 1 or the nucleotide sequence of nucleotides 444 to 1118 of SEQ ID NO:
- 1 or nucleotides 456 to 1118 of SEQ ID NO:
- 1, and encodes a protein having aldolase activity, wherein said stringent conditions comprise a wash step in 0.1×SSC and 0.1% SDS at 65° C.
- (c) a polynucleotide that encodes a protein comprising the amino acid sequence of SEQ ID NO:
- 2 or the amino acid sequence of residue numbers 5 to 225 of SEQ ID NO:
- 2;
- and
- (d) a polynucleotide that encodes a protein having an amino acid sequence that contains a substitution, deletion, insertion, or addition of one to ten amino acid residues in the amino acid sequence of SEQ ID NO:
- 2 or the amino acid sequence of residue numbers 5 to 225 of SEQ ID NO:
- 2, and has aldolase activity.
12 more elements
Dependencies are inferred from the claim text.