Cell-free extracts and synthesis of active hydrogenase
AssigneeThe Board of Trustees of the Leland Stanford Junior University
InventorsJames Robert Swartz, Marcus Emil Boyer, James Alan Stapleton, Alfred M. Spormann, Chia-Wei Wang
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Abstract
Enzymatically active hydrogenase is synthesized in a cell-free reaction. The hydrogenases are synthesized in a cell-free reaction comprising a cell extract derived from microbial strains expressing at least one hydrogenase accessory protein. In some embodiments, the extracts are produced under anerobic conditions.
Claims11
S30 extract of an E. Coili bacterial cell expressing at least one hydrogenase accessory protein encoding gene obtained from Shewanella oneidensis wherein said gene is selected from the group consisting of HydE, HydF and HydG genes.
Dependencies are inferred from the claim text.