Mouse cytokine receptor Zcytor16
AssigneeZymoGenetics, Inc.
InventorsScott R. Presnell, Wenfeng Xu, Wayne Kindsvogel, Zhi Chen
Life of this patent
Estimating the term…
Abstract
Cytokines and their receptors have proven usefulness in both basic research, animal models, and as therapeutics. The present invention provides a new cytokine receptor designated as “mouse Zcytor16,” which can bind and antagonize the IL-TIF cytokine.
Claims33
An isolated polynucleotide encoding a polypeptide comprising the sequence of amino acid residues selected from the group consisting of:
- (a) amino acid residues 24 to 230 of SEQ ID NO:48;
- and
- (b) amino acid residues 1 to 230 of SEQ ID NO:48.
An isolated polynucleotide that encodes a soluble cytokine receptor polypeptide comprising the sequence of amino acid residues from amino acid 24 to 230 of SEQ ID NO:48,
- wherein the soluble cytokine receptor polypeptide encoded by the polynucleotide sequence binds IL-10-Related T Cell-Derived inducible Factor (IL-TIF) or antagonizes IL-TIF activity.
An isolated polynucleotide that encodes a soluble cytokine receptor polypeptide comprising the sequence of amino acid residues as shown from amino acid 24 to 230 of SEQ ID NO:48, wherein the soluble cytokine receptor polypeptide encoded by the polynucleotide comprises a homodimeric, heterodimeric or multimeric receptor complex.
An expression vector comprising the following operably linked elements:
- (a) a transcription promoter;
- a first DNA segment encoding a soluble cytokine receptor polypeptide having an amino acid sequence as shown from amino acid 24 to 230 of SEQ ID NO:48;
- and a transcription terminator;
- and
- (b) a second transcription promoter;
- a second DNA segment encoding a soluble Class I or Class II cytokine receptor polypeptide;
- and a transcription terminator;
- and
- wherein the first and second DNA segments are contained within a single expression vector or are contained within independent expression vectors.
A DNA construct encoding a fusion protein comprising:
- a first DNA segment encoding a polypeptide having the sequence of amino acid residues as shown from amino acid 24 to 230 of SEQ ID NO:48;
- and
- at least one other DNA scgment encoding a soluble Class I or Class II cytokine receptor polypeptide,
- wherein the first and other DNA segments are connected in-frame;
- and
- wherein the first and other DNA segments encode the fusion protein.
Dependencies are inferred from the claim text.