Methods of modulating tubulin deacetylase activity
AssigneeThe Regents of the University of California
InventorsEric M. Verdin, Brian J. North, Scott M. Ulrich
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Abstract
The present invention provides methods for identifying agents that modulate a level or an activity of tubulin deacetylase polypeptide, as well as agents identified by the methods. The invention further provides methods of modulating tubulin deacetylase activity in a cell. The invention further provides methods of modulating cellular proliferation by modulating the activity of tubulin deacetylase.
Claims6
An in vitro method of identifying an agent that modulates an enzymatic activity of a human sirtuin type 2 (SIRT2) polypeptide that exhibits tubulin deacetylase activity, the method comprising:
- contacting a human SIRT2 polypeptide with a test agent in an assay mixture that comprises NAD and an acetylated tubulin peptide, wherein the acetylated tubulin peptide comprises amino acids PSD(AcK)TIG of SEQ ID NO:08;
- and
- determining the effect, if any, of the test agent on the tubulin deacetylase activity of the human SIRT2 polypeptide.
An in vitro method of identifying an agent that modulates an enzymatic activity of a human tubulin deacetylase, the method comprising:
- contacting a polypeptide comprising SEQ ID NO:2 with a test agent in an assay mixture that comprises NAD and an acetylated peptide comprising MPSD(AcK)TIGG (SEQ ID NO:08);
- and
- evaluating the acetylation state of the peptide in presence and absence of the test agent, wherein a change in the acetylation state of the peptide indicates that the test agent modulates enzymatic activity of the human tubulin deacetylase.
Dependencies are inferred from the claim text.