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US 7,351,537 B2Granted
Variants of cyan fluorescent protein with improved fluorescent properties
AssigneeVanderbilt University
InventorsDavid Piston, Mark Rizzo
Life of this patent
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Abstract
The present invention provide optimized cyan fluorescent protein (oCFP) variants. In particular, the variants exhibit increase quantum yield of fluorescence and a single component fluorescence lifetime.
Claims12
A nucleic acid encoding a modified fluorescent protein, wherein said modified fluorescent protein comprises the sequence of SEQ ID NO:
- 58, with the following modifications:
- an amino acid other than Y at position 145, a substitution of phenylalanine at position 64 with leucine, a substitution of serine at position 65 with threonine, a substitution of tyrosine at position 66 with tryptophan, a substitution of asparagine at position 146 with isoleucine, a substitution of methionine at position 153 with threonine, a substitution of valine at position 163 with alanine, a substitution of histidine at position 231 with leucine, and an addition of valine between positions 1 and 2.
An isolated host cell comprising nucleic acid encoding a modified fluorescent protein, wherein said “modified fluorescent protein comprises the sequence of SEQ ID NO:
- 58, with the following modifications:
- an amino acid other than Y at position 145, a substitution of phenylalanine at position 64 with leucine, a substitution of serine at position 65 with threonine, a substitution of tyrosine at position 66 with tryptophan, a substitution of asparagine at position 146 with isoleucine, a substitution of methionine at position 153 with threonine, a substitution of valine at position 163 with alanine, a substitution of histidine at position 231 with leucine, and an addition of valine between positions 1 and 2.
Dependencies are inferred from the claim text.