In vitro method for disassmbly/reassembly of papillomavirus virus-like particles (VLPs). Homogeneous VLP and cavsomere compositions produced by said methods: use thereof as vehicle for improved purification, and delivery of active agents
AssigneeMedImmune, LLC
InventorsMichael P. McCarthy, JoAnne A. Suzich
Life of this patent
Estimating the term…
Abstract
A method of disassembly/reassembly of papillomavinis VLPs is provided. The resultant VLPs have enhanced homogeneity, present conformational, neutralizing PV epitopes, and therefore are useful prophylactic and diagnostic agents. Further, these VLPs can be used to encapsulate desired moieties, e.g., therapeutic or diagnostic agents, or marker” DNAs, and the resultant VLPs used as in vivo delivery vehicles or as pseudovirions for evaluating vaccine efficacy.
Claims15
A method of producing purified human papillomavirus (HPV) virus-like particles (VLPs) comprising:
- purifying a recombinantly expressed HPV L1 protein or truncated version thereof in the presence of at least one reducing agent that maintains said recombinantly expressed HPV L1 protein or truncated version thereof in a form other than a VLP;
- and
- assembling said recombinantly expressed HPV L1 protein or truncated version thereof into purified human papillomavirus virus-like particles (VLPs).
A method of producing purified human papillomavirus (HPV) virus-like particles (VLPs), comprising:
- purifying a recombinantly expressed HPV L1 protein or truncated version thereof in the presence of at least one reducing agent that maintains said recombinantly expressed HPV L1 protein or truncated version thereof in a form other than a VLP;
- and
- assembling said recombinantly expressed HPV L1 protein or truncated version thereof into purified human papillomavirus virus-like particles (VLPs) by removing or oxidizing said at least one reducing agent.
Dependencies are inferred from the claim text.