Immunizing fish against viral infection
AssigneesYissum Research Development Company of the Hebrew University of Jerusalem, Ministry of Agriculture and Rural Development Dept. of Fisheries and Aquaculture
InventorsMoshe Kotler, Arnon Dishon, Janette Bishara Shieban, Maya Ilouze, Izhak Bejerano, Ayana Benet, Nissim Chen, Marina Hutoran, Ariel Ronen
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Abstract
The present invention provides the isolated causative agent of the disease affecting Cyprinus carpino and a method for its isolation. The invention further provides avirulent forms, e.g., live-attenuated form, inactivated form and genetically modified forms of the virus which may be used for vaccination of susceptible fish.
Claims20
An isolated avirulent form of a CV DNA virus that causes viral disease in fish, the CV DNA virus being a double stranded DNA virus having a capsid with a icosahedron morphology, the capsid being of about 90-110 nm in size as determined by electron microscopy, the DNA of the CV DNA virus having between about 250,000 and 300,000 base pairs.
An isolated avirulent form of a CV DNA virus that causes viral disease in fish, the CV DNA virus being a double stranded DNA virus having a capsid with a icosahedron morphology, the capsid being of about 90-110 nm in size as determined by electron microscopy, the DNA of the CV DNA virus having between about 260,000 and 285,000 base pairs.
An isolated avirulent form of a CV DNA virus that causes viral disease in fish, the CV DNA virus being a double stranded DNA virus having a capsid with a icosahedron morphology, the capsid being of about 90-110 nm in size as determined by electron microscopy, the DNA of the CV DNA virus having about 277,000 base pairs.
A method for the isolation of a live-attenuated carp DNA virus, comprising seeding of a virulent virus which causes viral disease in fish in fish cell culture, said virulent virus being a double stranded DNA virus having a capsid with a icosahedron morphology, the capsid being of about 90-110 nm in size as determined by electron microscopy, the DNA of the virus having between about 250,000 and 300,000 base pairs, identifying plaques caused by a virus with a reduced virulence and isolating the progeny virus from such a plaque.
An isolated live-attenuated CV DNA virus obtained by the method, comprising:
- seeding of a virulent virus which causes viral disease in fish cell culture, said virulent virus being a double stranded DNA virus having a capsid with a icosahedron morphology, the capsid being of about 90-110 nm in size as determined by electron microscopy, the DNA of the virus having between about 250,000 and 300,000 base pairs;
- identifying plaques caused by a virus with a reduced virulence;
- and
- isolating the progeny virus from such a plaque.
Dependencies are inferred from the claim text.