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US 7,351,408 B2Granted
Glycoconjugates and methods
AssigneeThe Regents of the University of California
InventorsCarolyn C. Bertozzi, Kevin J. Yarema, Lara K. Mahal
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AssigneeThe Regents of the University of California
InventorsCarolyn C. Bertozzi, Kevin J. Yarema, Lara K. Mahal
Estimating the term…
Methods for making the functionalized glycoconjugates include (a) contacting a cell with a first monosaccharide, and (b) incubating the cell under conditions whereby the cell (i) internalizes the first monosaccharide, (ii) biochemically processes the first monosaccharide into a second saccharide, (iii) conjugates the saccharide to a carrier to form a glycoconjugate, and (iv) extracellularly expresses the glycoconjugate to form an extracellular glycoconjugate comprising a selectively reactive functional group. Methods for forming products at a cell further comprise contacting the functional group of the extracellularly expressed glycoconjugate with an agent which selectively reacts with the functional group to form a product. Subject compositions include cyto-compatible monosaccharides comprising a nitrogen or ether linked functional group selectively reactive at a cell surface and compositions and cells comprising such saccharides.
A cell comprising an extracellular, plasma membrane associated cyto-compatible glycoconjugate comprising a monosaccharide selected from the group consisting of a ketone modified fucoside and a ketone modified sialoside having either a nitrogen or an ether linked functional group selectively reactive at a cell surface, wherein said cell is a eukaryotic cell and wherein said functional group is a ketone.
A cell comprising an extracellular, plasma membrane associated cyto-compatible glycoconjugate comprising a functionality at a cell surface, wherein said cell is a eukaryotic cell, wherein said functionality is in a ketone modified fucoside and a ketone modified sialoside further comprising said ketone reacted with hydrazide to form hydrazone.
A cell comprising an extracellular, plasma membrane associated cyto-compatible glycoconjugate comprising a monosaccharide having a functional group at a cell surface, wherein said cell is a eukaryotic cell, prepared according to the following process:
Dependencies are inferred from the claim text.