Self-contained microelectrochemical bioassay platforms and methods
AssigneeBoard of Trustees of the University of Arkansas
InventorsIngrid Fritsch, Robert Beitle, Jr., Zoraida Aguilar
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Abstract
Methods and devices for improved chemical and biomass detection assays combined well defined microstructures having independently addressable electrodes with various surface immobilization electrochemical assays. Combining known chemical detection immobilization assays, electrochemically active moieties with microstructures having independently addressable electrodes provides for vastly improved methods of detecting microorganisms, chemical compounds, and measuring membrane transport.
Claims37
A microassay structure comprising:
- at least one three-dimensional microstructure having a rigid or flexible substrate and a plurality of alternating conducting layers and insulating layers, wherein said conducting layers comprise a plurality of integrated, independently addressable electrodes sandwiched between said insulating layers;
- an analyte binding material attached to at least one of said conducting layers or said insulating layers and wherein said substrate comprises materials selected from the group consisting of a silicon wafer, ceramic or glass.
A microassay structure comprising:
- at least one three-dimensional microstructure having a substrate and a plurality of alternating conducting layers and insulating layers, between them, wherein said conducting layers comprise a plurality of integrated, independently addressable electrodes sandwiched between said insulating layers, wherein at least one of said electrodes is a bottom layer of said microstructure;
- and
- an analyte binding material tethered to said electrode on said bottom layer of said microstructure.
A microassay structure comprising:
- at least one three-dimensional microstructure comprising a substrate and a plurality of alternating conducting layers and insulating layers deposited on said substrate;
- at least one microcavity in said microstructure, wherein said alternating conducting layers and insulating layers form at least one wall of said microcavity;
- an analyte binding material attached to at least one of said conducting layers or said insulating layers at said at least one wall of said microcavity;
- and
- wherein said conducting layers comprise a plurality of integrated, independently addressable electrodcs sandwiched between said insulating layers.
Dependencies are inferred from the claim text.