Polynucleotides and polypeptides in plants

An expression vector for expression of a polypeptide that confers greater cold tolerance in a transgenic plant than the cold tolerance of a control plant wherein the expression vector comprises:

• (1) a constitutive, inducible, or tissue-specific promoter;
• and
• (2) a recombinant polynucleotide encoding the polypeptide that regulates transcription, wherein the polypeptide comprises a conserved Myb DNA-binding domain having at least 95% sequence identity with the conserved Myb DNA-binding domain of amino acid coordinates 13-116 of SEQ ID NO:
• 438 and wherein the conserved Myb DNA-binding domain binds DNA;
• wherein the constitutive, inducible, or tissue-specific promoter regulates expression of the polypeptide.

A method for producing a transgenic plant having greater tolerance to cold as compared to a control plant as the result of expressing a polypeptide that regulates transcription in the transgenic plant, wherein the control plant is without the recombinant polynucleotide, the method comprising:

• (a) providing an expression vector encoding the polypeptide, wherein the polypeptide comprises a conserved Myb DNA-binding domain having at least 95% sequence identity with the conserved Myb DNA-binding domain of amino acid coordinates 13-116 of SEQ ID NO:
• 438 and wherein the conserved Myb DNA-binding domain binds DNA;
• and
• (b) introducing the expression vector into a first target plant to produce the transgenic plant, or introducing the expression vector into a cell of a second target plant, and growing the cell into the transgenic plant.

A transgenic seed comprising an expression vector for expression of a polypeptide that confers greater cold tolerance in a transgenic plant relative to a control plant, wherein the expression vector comprises:

• (1) a constitutive, inducible, or tissue-specific promoter;
• and
• (2) a recombinant polynucleotide encoding the polypeptide that regulates transcription, wherein the polypeptide comprises a conserved Myb DNA-binding domain having at least 95% sequence identity with the conserved Myb DNA-binding domain of amino acid coordinates 13-116 of SEQ ID NO:
• 438 and wherein the conserved Myb DNA-binding domain binds DNA;
• and wherein the constitutive, inducible, or tissue-specific promoter regulates expression of the polypeptide.

A method for producing a transgenic plant having greater tolerance to cold as compared to a control plant as the result of expressing a polypeptide that regulates transcription in the transgenic plant, wherein the control plant is without the recombinant polynucleotide, the method comprising:

• (a) providing an expression vector encoding a polypeptide comprising the Myb DNA-binding domain of amino acid coordinates 13-116 of SEQ ID NO:
• 438 and wherein the conserved Myb DNA-binding domain binds DNA;
• and
• (b) introducing the expression vector into a plant or a cell of a plant, thereby producing the transgenic plant.