Production of flavonoids by recombinant microorganisms
AssigneeThe Research Foundation of State University of New York
InventorsMattheos Koffas, Effendi Leonard, Yajun Yan, Joseph Chemler
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Abstract
Methods and compositions are provided for production of flavonoids in microbial hosts. The compositions comprises a set of genes which encode for enzymes involved in one or more steps in the biosymthetic pathway for the conversion of phenylpropanoids to various flavonoids. The method comprises the steps of introducing the set of genes into a heterologous host cell, allowing growth of the cells in a suitable medium such that the expression of the genes results in production of enzymes. When specific substrate(s) is/are provided to the transformed cell, the enzymes act on the substrate(s) to produce the desired flavonoids.
Claims3
A method of producing a flavonoids in a host cell comprising the steps of:
- a) introducing into the host cell an exogenous nucleic acid comprising a set of genes encoding for enzymes sufficient to direct the synthesis of a desired flavonoid from a substrate, wherein the set of genes comprises Malus domestica Flavanone 3-hydroxylase (FHT), Dianthus gratianovolitanus or Anthurium andraeanum Dihydroflavonol 4-reductase (DFR), Malus domestica Anthocyanidin synthase (ANS) and Petunia x hybrida UDP-glucose:flavonoid 3-O-glucosyltransferase (3-GT);
- b) providing the substrate to the cells, wherein the substrate is a flavanone;
- c) culturing the cell under conditions permitting the synthesis of the flavonoid by the cells;
- and
- d) isolating said flavonoids from said cell, wherein the flavonoids is an anthocyanidin selected from the group of anthocyanidins consisting of pelargonidin 3-O-glucoside and cyanidin 3-O-glucoside.
Dependencies are inferred from the claim text.