US 7,338,778 B2 · Luminescent method for determining cell oxygen consumption

Abstract

The present invention relates to methods for detection and evaluation of metabolic activity of eukaryotic and/or prokaryotic cells based upon their ability to consume dissolved oxygen. The methods utilize a luminescence detection system which makes use of the sensitivity of the luminescent emission of certain compounds to the presence of oxygen, which quenches (diminishes) the compound's luminescent emission in a concentration dependent manner. Respiring eukaryotic and/or prokaryotic cells will affect the oxygen concentration of a liquid medium in which they are immersed. Thus, this invention provides a convenient system to gather information on the presence, identification, quantification and cytotoxic activity of eukaryotic and/or prokaryotic cells by determining their effect on the oxygen concentration of the media in which they are present.

Claims15

Dependent claims

A method for determining oxygen consumption by cells comprising the steps of:

providing a sample comprising a mammalian cell culture;
placing the sample in chemical communication with a sensor composition which comprises a luminescent compound that exhibits a change in luminescent property, upon exposure to oxygen, wherein the presence of the sensor composition is nondestructive to the mammalian cells;
irradiating the sensor composition with light containing wavelengths which cause the luminescent compound to luminescence;
and
determining the change in luminescent property, over a period of time, wherein the change in luminescent property is indicative of a change in oxygen consumption by the mammalian cells.

2 more elements

Dependencies are inferred from the claim text.

Facts

Application

10/957,802Filed 4 Oct 2004 · division

Priority

18 Apr 1991

Published

US 2005/0191613 A11 Sep 2005

Granted

4 Mar 2008

Term ends

Not estimatedThe source records don’t support an estimate.

Classification6 codes

C12Q 1/18
Involving viable microorganisms: testing for antimicrobial activity of a material
C12Q 1/04
Involving viable microorganisms: determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides;…
C12Q 2304/44
Detection of gases: oxygen
Y02A 90/10
Technologies having an indirect contribution to adaptation to climate change: information and communication technologies [ICT] supporting…

Family

Application07/687,359Parent of this continuation-in-part · filed 18 Apr 1991

Application08/025,899Parent of this continuation-in-part · filed 3 Mar 1993

Application08/715,557Parent of this continuation-in-part · filed 18 Sep 1996

Application09/342,720Parent of this division · filed 29 Jun 1999

Application10/116,777Parent of this division · filed 4 Apr 2002

US 2005/0191613 A1Pre-grant publication · published 1 Sep 2005

US 6,900,030 B2Parent of this division

US 6,395,506 B1Parent of this division

Application09/342,720Parent of this continuation-in-part

US 5,567,598 AParent of this continuation-in-part

US 2002/0155424 A1Published application

US 7,338,778 B2This patent

From the US filing history. Foreign family members aren’t listed.

Luminescent method for determining cell oxygen consumption

Life of this patent

Luminescent method for determining cell oxygen consumption

Life of this patent

Abstract

Claims15