Irreversible immobilization of enzymes into polyurethane coatings
AssigneesUniversity of Pittsburgh, Bayer Material Science AG
InventorsAlan J. Russell, Geraldine F. Drevon, Douglas A. Wicks, Karsten Danielmeier
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Abstract
A method of irreversibly immobilizing an enzyme in a polyurethane and an enzyme-containing polyurethane having a degree of immobilization of the enzyme of approximately 100%. The synthesis of waterborne polyurethanes in the presence of enzyme has enabled the irreversible attachment of the enzyme to the polymeric matrix. The distribution of immobilized enzyme as well as activity retention are homogeneous within the polyurethane. Decreasing ECC hydrophobicity, via the use of a less hydrophobic polyisocyanate prepolymer during polymerization, significantly enhanced the intrinsic activity of the ECC.
Claims20
A method of irreversibly immobilizing an enzyme into a polyurethane material comprising the steps of:
- (a) reacting a mixture of (i) a polyol dispersion coreactant, and (ii) the enzyme to create an aqueous mixture;
- (b) adding (iii) a water-dispersible polyisocyanate to the aqueous mixture,
- (c) allowing the polyisocyanate to disperse in the aqueous mixture to produce a dispersion;
- (d) applying the dispersion onto a substrate;
- and
- (e) curing the enzyme-containing dispersion.
A method of irreversibly immobilizing diisopropylfluorophosphatase into a polyurethane comprising the steps of:
- (a) reacting a mixture of (i) a polyol dispersion coreactant having a water content of 70 wt. %, (ii) a polyether- modified polydimethyl siloxane surfactant, (iii) a buffered medium comprising bis-tris propane buffer and CaCl2 and (iv) diisopropylfluorophosphatase to create an aqueous mixture;
- (b) adding a polyisocyanate to the aqueous mixture to produce a diisopropylfluorophosphatase dispersion;
- (c) applying the diisopropylfluorophosphatase dispersion onto a substrate;
- and
- (d) curing the diisopropylfluorophosphatase dispersion to form a polyurethane.
Dependencies are inferred from the claim text.