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US 7,326,681 B2Granted
Methods for producing modified glycoproteins
AssigneeGlycoFi, Inc.
InventorTillman U. Gerngross
Life of this patent
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AssigneeGlycoFi, Inc.
InventorTillman U. Gerngross
Estimating the term…
Cell lines having genetically modified glycosylation pathways that allow them to carry out a sequence of enzymatic reactions, which mimic the processing of glycoproteins in humans, have been developed. Recombinant proteins expressed in these engineered hosts yield glycoproteins more similar, if not substantially identical, to their human counterparts. The lower eukaryotes, which ordinarily produce high-mannose containing N-glycans, including unicellular and multicellular fungi are modified to produce N-glycans such as Man5GlcNAc2 or other structures along human glycosylation pathways. This is achieved using a combination of engineering and/or selection of strains which: do not express certain enzymes which create the undesirable complex structures characteristic of the fungal giycoproteins, which express exogenous enzymes selected either to have optimal activity under the conditions present in the fungi where activity is desired, or which are targeted to an organelle where optimal activity is achieved, and combinations thereof wherein the genetically engineered eukaryote expresses multiple exogenous enzymes required to produce “human-like” glycoproteins.
A composition comprising isolated recombinant glycoproteins, wherein 50% to 100% of the glycoproteins comprise an asparagine-linked oligosaccharide structure selected from the group consisting of the oligosaccharide structures GlcNAc(2-4)Man3GlcNAc2.
A composition comprising isolated recombinant glycoproteins, wherein 50% to 100% of the glycoproteins comprise an asparagine-linked oligosaccharide structure selected from the group consisting of the oligosaccharide structures Gal(1-4)GlcNAc(2-4)Man3GlcNAc2.
A composition comprising isolated recombinant glycoproteins, wherein 50% to 100% of the glycoproteins comprise an asparagine-linked oligosaccharide structure selected from the group consisting of the oligosaccharide structures NANA(1-4)Gal(1-4)GlcNAc(2-4)Man3GlcNAc2.
A composition comprising isolated recombinant glycoproteins, wherein 50% to 100% of the glycoproteins comprise an asparagine-linked oligosaccharide structure selected from the group consisting of the oligosaccharide structures GlcNAc2 having fewer than 4 mannose residues and optionally one or more terminal sugar residues.
A composition comprising isolated recombinant glycoproteins, wherein 50% to 100% of the glycoproteins comprise an asparagine-linked oligosaccharide structure selected from the group consisting of the oligosaccharide structures Man3GlcNAc2 and Man3GlcNAc2 having one or more terminal sugar residues.
A composition comprising isolated recombinant glycoproteins, wherein 50% to 100% of the glycoproteins comprise an asparagine-linked oligosaccharide structure consisting of GlcNAc(2)Man3GlcNAc2.
A composition comprising isolated recombinant glycoproteins, wherein 50% to 100% of the glycoproteins comprise an asparagine-linked oligosaccharide structure consisting of GlcNAc(3)Man3GIcNAc2.
A composition comprising isolated recombinant glycoproteins, wherein 50% to 100% of the glycoproteins comprise an asparagine-linked oligosaccharide structure consisting of GlcNAc(4)Man3GlcNAc2.
Dependencies are inferred from the claim text.