Methods for detecting Corpuscles of Stannius protein stanniocalcin
AssigneeHuman Genome Sciences, Inc.
InventorsHenrik S. Olsen, Mark D. Adams
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Abstract
The present invention relates to human Corpuscles of Stannius polypeptides and DNA (RNA) encoding such polypeptides. Also provided is a procedure for producing such polypeptides by recombinant techniques and antagonists against such polypeptides. Human Corpuscles of Stannius protein inhibits calcium uptake and reduces renal excretion of phosphate. Also provided is a diagnostic assay to detect mutations in the nucleic acid sequence encoding the polypeptide and for altered concentrations of the polypeptide in a sample derived from a host.
Claims14
A method for detecting the presence of a polypeptide in a sample derived from a human comprising measuring the level of said polypeptide in said sample, wherein said polypeptide is selected from the group consisting of:
- (i) a polypeptide having the deduced amino acid sequence of SEQ ID NO:2 and fragments thereof;
- and
- (ii) a polypeptide encoded by the cDNA of ATCC Deposit No. 75652 and fragments thereof.
A method for detecting a polypeptide in a sample derived from a human comprising:
- (a) contacting said sample with an antibody specific to the polypeptide to form a complex;
- and
- (b) detecting said complex
- wherein said polypeptide is selected from the group consisting of:
- (i) a polypeptide having the deduced amino acid sequence of SEQ ID NO:2 and fragments thereof;
- and
- (ii) a polypeptide encoded by the cDNA of ATCC Deposit No. 75652 and fragments
- thereof.
A method for detecting over-expression of a polypeptide in a sample derived from a human comprising:
- (a) detecting the level of said polypeptide in a sample derived from a human;
- and
- (b) comparing the detected level in step (a) to a normal control-sample, wherein an increased detected level in step (a) as compared to the normal control is indicative of over-expression of said polypeptide in said sample,
- wherein said polypeptide is selected from the group consisting of:
- (i) a polypeptide having the deduced amino acid sequence of SEQ ID NO:2 and fragments thereof;
- and
- (ii) a polypeptide encoded by the cDNA of ATCC Deposit No. 75652 and fragments
- thereof.
Dependencies are inferred from the claim text.