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US 7,323,553 B2Granted
Non-affinity purification of proteins
AssigneeGenentech, Inc.
InventorsRobert Fahrner, Deborah Follman, Benedicte Lebreton, Robert van Reis
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Abstract
The present invention relates to a method for protein purification that involves the combination of non-affinity chromatography with HPTFF.
Claims25
A method for purifying a target protein from a mixture containing a host cell protein, consisting essentially of subjecting said mixture to:
- (a) a first non-affinity purification step, and
- (b) a second non-affinity purification step, followed by
- (c) high-performance tangential-flow filtration (HPTFF), and
- (d) isolating said protein in a purity containing less than 100 parts per million (ppm) of said host cell protein, wherein said method includes no affinity purification step.
1 more element
Dependencies are inferred from the claim text.