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US 7,320,862 B2Granted
Microfluidic extraction method
OwnerCord F. Stähler, Peer F. Stähler +1inventors
InventorsCord F. Stähler, Peer F. Stähler, Manfred Müller
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Abstract
The invention relates to an extraction method for isolating target molecules from a sample using a microfluidic carrier.
Claims30
A method for selectively isolating, in parallel, a plurality species of target molecules from a sample, comprising the steps:
- (a) providing a microfluidic carrier having an array of a plurality of different receptors which are immobilized on or in the carrier wherein each different receptor is immobilized at a different position,
- (b) directing a sample comprising target molecules to be selectively isolated in parallel through said microfluidic carrier under conditions under which said target molecules can specifically bind to the receptors immobilized on the carrier,
- (c) removing unbound material of the sample from the carrier, and
- (d) isolating, in parallel, the target molecules bound to the carrier by selective elution of said species of target molecules.
1 more element
A method for selectively isolating in parallel, several species of target molecules from a sample, comprising the steps:
- (a) providing a microfluidic carrier having an array of a plurality of different receptors which are immobilized on or in the carrier wherein each different receptor is immobilized at a different position,
- (b) directing a sample comprising target molecules to be selectively isolated in parallel through said microfluidic carrier under conditions under which interfering components of a sample can specifically bind to the receptors immobilized on the carrier, and
- (c) isolating the unbound target molecules from the carrier by elution.
A method for selectively isolating, in parallel, a plurality species of target molecules from a sample, comprising the steps:
- (a) providing a microfluidic carrier,
- (b) synthesizing in situ on said microfluidic carrier an array of a plurality of different receptors, immobilized on or in the carrier, wherein each different receptor is immobilized at a different position,
- (c) directing a sample comprising target molecules to be selectively isolated in parallel through said microfluidic carrier under conditions under which said target molecules can specifically bind to the receptors immobilized on the carrier,
- (d) removing unbound material of the sample from the carrier, and
- (e) isolating, in parallel, the target molecules bound to the carrier by selective elution of said species of target molecules.
2 more elements
A method for isolating nucleic acid analytes comprising the steps:
- (a) providing a microfluidic carrier,
- (b) synthesizing in situ on said microfluidic carrier an array of a plurality of different receptors, immobilized on or in the carrier, wherein each different receptor is immobilized at a different position,
- (c) directing a sample comprising target molecules to be selectively isolated in parallel through said microfluidic carrier under conditions under which said target molecules can specifically bind to the receptors immobilized on the carrier,
- (d) removing unbound material of the sample from the carrier, and
- (e) isolating, in parallel, the target molecules bound to the carrier by selective elution of said species of target molecules.
2 more elements
Dependencies are inferred from the claim text.