US 7,316,925 B2 · Codon optimized synthetic plasmids

Abstract

One aspect of the current invention is an optimized synthetic mammalian expression plasmid (e.g. pAV0201). This new plasmid comprise a therapeutic element, and a replication element. The therapeutic element of the new plasmid comprises a eukaryotic promoter; a 5′ untranslated region (“UTR”); a codon-optimized-eukaryotic therapeutic gene sequence; and a poly adenylation signal. The therapeutic elements of this plasmid are operatively linked and located in a first operatively-linked arrangement. Additionally, the optimized synthetic mammalian expression plasmid comprises replication elements, wherein the replication elements are operatively linked and located in a second operatively-linked arrangement. The replication elements comprise a selectable marker gene promoter, a ribosomal binding site, and an origin of replication. The first-operatively-linked arrangement and the second-operatively-linked arrangement comprise a circular structure of the codon optimized synthetic mammalian expression plasmid.

Claims2

Dependent claims

A synthetic mammalian expression plasmid for plasmid mediated gene supplementation in a species comprising:

(a) a synthetic or eukaryotic promoter comprising SEQ ID NO:
15;
(b) a codon-optimized eukaryotic therapeutic gene sequence comprising SEQ ID NO:
7;
(c) a polyadenylation signal;
(d) a selectable marker gene sequence;
(e) a ribosomal binding site;
(f) a promoter for the selectable marker gene sequence;
and
(g) an origin of replication;
wherein the synthetic or eukaryotic promoter, the codon-optimized-eukaryotic therapeutic gene sequence, and the polyadenylation signal comprise therapeutic elements of the synthetic mammalian expression plasmid;
the therapeutic elements are operatively linked and located in a first operatively-linked arrangement;
the selectable marker gene promoter, the ribosomal binding site, the selectable marker gene sequence, and the origin of replication comprise replication elements of the synthetic mammalian expression plasmid;
the replication elements are operatively linked and located in a second-operatively-linked arrangement;
and the first-operatively linked arrangement and the second-operatively-linked arrangement comprise a circular structure of the synthetic mammalian expression plasmid.

12 more elements

Dependencies are inferred from the claim text.

Codon optimized synthetic plasmids

Life of this patent

Codon optimized synthetic plasmids

Life of this patent

Abstract

Claims2