Recombinant sendai virus vector for introducing exogenous genes to airway epithelia
AssigneeDNAVEC Research Inc.
InventorsYoshikazu Yonemitsu, Mamoru Hasegawa, Eric W F W Alton
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Abstract
Provided are a recombinant Sendai virus vector for introducing exogenous genes to airway epithelia and a method for introducing exogenous genes using the vector. The recombinant Sendai virus vector enables efficient gene transfer to native mucus-layered airway epithelial cells by briefly contacting the vector with the cells. Furthermore, the vector can introduce genes to not only apical surfaces but also submucosal glands where CFTR primarily expresses. The vector can thus be used for gene therapy of CF, a CFTR-deficient disease.
Claims17
A composition comprising a recombinant Sendai virus vector carrying in its genome a cystic fibrosis transmembrane conductance regulator (CFTR) gene, wherein the Sendai virus vector does not contain at least one gene selected from the group consisting of F gene, an HN gene, and an M gene.
A method for introducing an exogenous gene into airway epithelia, the method comprising contacting airway epithelia covered with mucus with a composition comprising a recombinant Sendai virus vector carrying an exogenous gene in its genome.
A method for expressing a cystic fibrosis transmembrane conductance regulator (CFTR) gene in an airway epithelial cell, said method comprising the step of
- introducing into said cell a recombinant Sendai virus vector carrying in its genome a cystic fibrosis transmembrane conductance regulator (CFTR) gene,
- wherein said CFTR gene is expressed in said cell.
A method for expressing an exogenous gene in an airway epithelial cell, said method comprising the step
- of introducing into said cell a recombinant Sendai virus vector carrying an exogenous gene in its genome,
- wherein said exogenous gene is expressed in said cell.
Dependencies are inferred from the claim text.