Tissue matrices comprising placental stem cells, and methods of making the same
AssigneeAnthrogenesis Corporation
InventorRobert J. Hariri
Life of this patent
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Abstract
A method of manufacturing a tissue matrix for implantation into a patient is disclosed. The method sets forth collecting embryonic stem cells from a placenta which has been treated to remove residual cord blood and seeding the collected stem cells onto or into a tissue matrix. The seeded tissue matrix is then implanted on or into a patient. The seeded tissue matrix made by the method of the present invention is also disclosed.
Claims5
A tissue matrix comprising placental stem cells, wherein the placental stem cells are CD10+, CD29+, CD34−, CD44+, CD45−, CD54+, CD90+, SH2+, SH3+, and SH4+;
- wherein said placental stem cells are additionally OCT-4+, SSEA3− or SSEA4−;
- and wherein said tissue matrix is made by a method comprising seeding human placental stem cells into or onto a decellularized or synthetic tissue matrix, wherein said stem cells are from a human placenta that has been drained of cord blood and perfused to remove residual blood.
A method of making a tissue matrix, comprising seeding human placental stem cells into or onto a decellularized or synthetic tissue matrix, wherein said stem cells are from a human placenta that has been drained of cord blood and perfused to remove residual blood, wherein said placental stem cells are CD34−, SSEA3−, SSEA4−, and OCT-4+.
A method of making a tissue matrix, comprising seeding human placental stem cells into or onto a decellularized or synthetic tissue matrix, wherein said stem cells are from a human placenta that has been drained of cord blood and perfused to remove residual blood, wherein said placental stem cells are CD10+, CD29+, CD34−, CD44−, CD45−, CD54+, CD90+, SH2+, SH3+, and SH4+;
- and wherein said placental stem cells are additionally OCT-4+, SSEA3− or SSEA4−.
A decellularized or synthetic tissue matrix comprising human placental stem cells, wherein the placental stem cells are CD34−, SSEA3−, SSEA4−, and OCT-4+.
A decellularized or synthetic tissue matrix comprising human placental stem cells, wherein the placental stem cells are CD34−, CD10+, CD29+, CD34−, CD44+, CD45−, CD54+, CD90+, SH2+, SH3+, and SH4+;
- and wherein said placental stem cells are additionally OCT-4+, SSEA3− or SSEA4−.
Dependencies are inferred from the claim text.