Back to results
US 7,309,605 B1Granted
Nucleotide sequence encoding the enzyme I-SceI and the uses thereof
AssigneesInstitut Pasteur, Universite Pierre et Marie Curie
InventorsBernard Dujon, Andre Choulika, Arnaud Perrin, Jean-Francois Nicolas
Life of this patent
Estimating the term…
Abstract
An isolated DNA encoding the enzyme I-SceI is provided. The DNA sequence can be incorporated in cloning and expression vectors, transformed cell lines and transgenic animals. The vectors are useful in gene mapping and site-directed insertion of genes.
Claims17
A method for inducing at least one site directed double-stranded break in the DNA of an organism comprising:
- (a) providing an isolated cell of said organism containing at least one Group I intron encoded endonuclease recognition site at a location in the DNA of the cell,
- (b) providing said Group I intron encoded endonuclease to said cell by genetically modifying the cell with a nucleic acid comprising said Group I intron encoded endonuclease or by introducing said Group I intron encoded endonuclease protein into the cell such that the Group I intron encoded endonuclease cleaves said Group I intron encoded endonuclease site at the location in the DNA of the cell.
Dependencies are inferred from the claim text.