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US 7,307,148 B2Granted
Method for purification of albumin conjugates
AssigneeConjuChem Biotechnologies Inc.
InventorsNathalie Bousquet-Gagnon, Omar Quraishi, Dominique P. Bridon
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Abstract
The present invention relates to a method for separating albumin conjugate from unconjugated albumin in a solution comprising albumin conjugate and unconjugated albumin by loading the solution onto a hydrophobic support equilibrated in aqueous buffer having a high salt content; applying to the support a gradient of decreasing salt concentration; and collecting the eluted albumin conjugate.
Claims28
A method for separating albumin conjugate from unconjugated albumin in a solution comprising albumin conjugate and unconjugated albumin, the method comprising:
- a) loading said solution onto a hydrophobic interaction chromatography matrix equilibrated in aqueous buffer at a salt concentration high enough to promote matrix-protein interactions;
- b) applying to said matrix a gradient of decreasing salt concentration;
- and
- c) collecting said albumin conjugate.
1 more element
Dependencies are inferred from the claim text.