Method for detecting carcinomas in a solubilized cervical body sample

A method for detecting cervical carcinomas, cervical intraepithelial neoplasms or cervical carcinomas in situ in a human subject, the method comprising the steps of:

• (a) obtaining a cervical body sample from the human subject,
• (b) solubilizing the cervical body sample in an aqueous lysis buffer comprising 0.1-1% SDS, and
• (c) reacting the solubilized cervical sample in the aqueous lysis buffer comprising 0.1-1% SDS with an antibody against cyclin dependent kinase inhibitor p16, and
• (d) determining the overexpression of cyclin dependent kinase inhibitor p16 in the solubilized cervical sample by comparing the level of cyclin dependent kinase inhibitor p16 within said solubilized cervical sample with the level present in a solubilized healthy human cervical sample, wherein cervical carcinomas, cervical intraepithelial neoplasms or cervical carcinomas in situ in the human subject are detected if overexpression of cyclin dependent kinase inhibitor p16 in the solubilized cervical sample is determined.