Herbicide-tolerant plants through bypassing metabolic pathway
AssigneeBayer CropScience AG
InventorsOlivier Zink, Eric Paget, Anne Rolland, Alain Sailland, Georges Freyssinet
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Abstract
DNA sequence of a gene of hydroxy-phenyl pyruvate dioxygenase and production of plants containing a gene of hydroxy-phenyl pyruvate dioxygenase and which are resistant to herbicides. DNA sequence of a gene of hydroxy-phenyl pyruvate dioxygenase; isolation from a bacteria or a plant; utilization for obtaining plants tolerant to herbicides.
Claims21
An isolated nucleic acid encoding an HPP oxidase, characterized in that it comprises a nucleic acid sequence selected from the group comprising the coding sequences of SEQ ID No. 1, SEQ ID No. 3, SEQ ID No. 5 and SEQ ID No. 15, or a nucleic acid capable of selectively hybridizing to the coding sequence of said SEQ ID No. 1, 3, 5 or 15 under the following conditions:
- prehybridization for four hours at 68° C. in a solution of 5×SSC, 6% Denhardt's, and 0.5% SDS;
- hybridization for sixteen hours at 68° C.;
- and washes at 65° C. in 0.5×SSC, 0.1% SDS.
A method of growing plants tolerant to a herbicide which is an HPPD inhibitor, comprising the step of growing plants expressing at least one enzyme insensitive to said herbicide that allows conversion of para-hydroxyphenylpyruvate (HPP) to 4-hydroxyphenyl-acetic acid (4-HPA) and at least one enzyme insensitive to said herbicide that allows conversion of 4-HPA to homogentisate as a result of nucleic acid encoding said enzymes being inserted into the genome of said plants, wherein said at least one enzyme that allows conversion of para-hydroxyphenylpyruvate (HPP) to 4-hydroxyphenyl-acetic acid (4-HPA) is HPP oxidase, wherein said HPP oxidase comprises an amino acid sequence selected from the group consisting of SEQ ID NOS:
- 2, 4, 6 and 16.
A method of growing plants tolerant to a herbicide which is an HPPD inhibitor, comprising the step of growing plants expressing at least one enzyme insensitive to said herbicide that allows conversion of para-hydroxyphenylpyruvate (HPP) to 4-hydroxyphenyl-acetic acid (4-HPA) as a result of nucleic acid encoding said enzyme being inserted into the genome of said plants, wherein said at least one enzyme that allows conversion of para-hydroxyphenylpyruvate (HPP) to 4-hydroxyphenyl-acetic acid (4-HPA) is HPP oxidase, wherein said HPP oxidase comprises an amino acid sequence selected from the group consisting of SEQ ID NOS:
- 2, 4, 6 and 16.
A method for making plants tolerant to a herbicide which is an HPPD inhibitor, comprising the steps of
- inserting a nucleic acid encoding at least one enzyme insensitive to said herbicide that allows conversion of para-hydroxyphenylpyruvate (HPP) to 4-hydroxyphenyl-acetic acid (4-HPA) and a nucleic acid encoding at least one enzyme insensitive to said herbicide that allows conversion of 4-HPA to homogentisate, wherein said at least one enzyme that allows conversion of para-hydroxyphenylpyruvate (HPP) to 4-hydroxyphenyl-acetic acid (4-HPA) is HPP oxidase, and wherein said HPP oxidase comprises an amino acid sequence selected from the group consisting of SEQ ID NOS:
- 2, 4, 6 and 16, into the genome of a plant cell;
- regenerating a plant from said plant cell;
- and
- growing said plant, wherein said at least one enzyme insensitive to said herbicide that allows conversion of para-hydroxyphenylpyruvate (HPP) to 4-hydroxyphenyl-acetic acid (4-HPA) and said at least one enzyme insensitive to said herbicide that allows conversion of 4-HPA to homogentisate are expressed in said plant.
A method for making plants tolerant to a herbicide which is an HPPD inhibitor, comprising the steps of
- inserting a nucleic acid encoding at least one enzyme insensitive to said herbicide that allows conversion of para-hydroxyphenylpyruvate (HPP) to 4-hydroxyphenyl-acetic acid (4-HPA), wherein said at least one enzyme that allows conversion of para-hydroxyphenylpyruvate (HPP) to 4-hydroxyphenyl-acetic acid (4-HPA) is HPP oxidase, and wherein said HPP oxidase comprises an amino acid sequence selected from the group consisting of SEQ ID NOS:
- 2, 4, 6 and 16, into the genome of a plant cell;
- regenerating a plant from said plant cell;
- and
- growing said plant, wherein said at least one enzyme insensitive to said herbicide that allows conversion of para-hydroxyphenylpyruvate (HPP) to 4-hydroxyphenyl-acetic acid (4-HPA) is expressed in said plant.
Dependencies are inferred from the claim text.