Biocompatible phase invertable proteinaceous compositions and methods for making and using the same
AssigneeMatrix Medical, LLC
InventorsKemal Schankereli, Ronald Dieck
Life of this patent
Estimating the term…
Abstract
Biocompatible phase invertable proteinaceous compositions and methods for making and using the same are provided. The subject phase invertable compositions are prepared by combining a proteinaceous substrate and a cross-linker. The proteinaceous substrate includes one or more proteins and an adhesion modifier, and may also include one or more of: a pasticizer, a carbohydrate, or other modification agent. In certain embodiments, the cross-linker is a heat-treated dialdehyde, e.g., heat-treated glutaraldehyde. Also provided are kits for use in preparing the subject compositions. The subject compositions, kits and systems find use in a variety of different applications.
Claims54
A fluid phase invertible composition that goes from a first fluid state to a second non-fluid state in which said composition is incapable of fluid flow, wherein said composition is produced by combining:
- (a) a fluid substrate comprising a proteinaceous material, wherein said proteinaceous material comprises one or more of:
- albumin, elastin, fibrin and soluble and insoluble forms of collagen and combinations thereof, and
- (b) a heat stabilized dialdehyde crosslinking agent that has been produced by maintaining an initial dialdehyde crosslinking composition at a temperature ranging from about 45 to about 55° C. for a period of time ranging from about 1 to about 20 days, wherein said heat stabilized crosslinking agent has been heat stabilized prior to contacting said substrate;
- wherein said fluid phase invertible composition goes from a first fluid state to a second non-fluid state in which said composition is incapable of fluid flow in a period of time ranging from about 10 seconds to about 10 minutes following crosslinking-of said substrate by said heat stabilized dialdehyde crosslinking agent.
A fluid phase invertible composition that goes from a first fluid state to a second non-fluid state in which said composition is incapable of fluid flow, wherein said composition is produced by combining:
- (a) a fluid substrate comprising:
- (i) a proteinaceous material, wherein said proteinaceous material comprises one or more of:
- albumin, elastin, fibrin and soluble and insoluble forms of collagen and combinations thereof, and
- (ii) an adhesion modifier;
- and
- (b) a heat stabilized dialdehyde crosslinking agent that has been produced by maintaining an initial dialdehyde crosslinking composition at a temperature ranging from about 45 to about 55° C. for a period of time ranging from about 1 to about 20 days, wherein said heat stabilized crosslinking agent has been heat stabilized prior to contacting said substrate;
- wherein said fluid phase invertible composition goes from a first fluid state to a second non-fluid state in which said composition is incapable of fluid flow in a period of time ranging from about 10 seconds to about 10 minutes following crosslinking of said substrate by said heat stabilized dialdehyde crosslinking agent.
A fluid phase invertible composition that goes from a first fluid state to a second non-fluid state in which said composition is incapable of fluid flow, wherein said composition is produced by combining:
- (a) a fluid substrate comprising:
- (i) a proteinaceous material, wherein said proteinaceous material comprises one or more of:
- albumin, elastin, fibrin and soluble and insoluble forms of collagen and combinations thereof;
- (ii) an adhesion modifier;
- and
- (iii) a plasticizer;
- and
- (b) a heat stabilized dialdehyde crosslinking agent that has been produced by maintaining an initial dialdehyde crosslinking composition at a temperature ranging from about 45 to about 55° C. for a period of time ranging from about 1 to about 20 days, wherein said heat stabilized crosslinking agent has been heat stabilized prior to contacting said substrate;
- wherein said fluid phase invertible composition is goes from a first fluid state to a second non-fluid state in which said composition is incapable of fluid flow in a period of time ranging from about 10 seconds to about 10 minutes following crosslinking of said substrate by said heat stabilized dialdehyde crosslinking agent.
A method comprising:
- (i) producing a fluid phase invertible composition in a step consisting essentially of mixing:
- (a) a fluid substrate comprising a proteinaceous material, wherein said proteinaceous material comprises one or more of:
- albumin, elastin, fibrin and soluble and insoluble forms of collagen and combinations thereof;
- and
- (b) a heat stabilized dialdehyde crosslinking agent that has been produced by maintaining an initial dialdehyde crosslinking composition at a temperature ranging from about 45 to about 55° C. for a period of time ranging from about 1 to about 20 days;
- to produce said fluid phase invertible composition that goes from a first fluid state to a second non-fluid state in which said composition is incapable of fluid flow in a period of time ranging from about 10 second to about 10 minutes following combination of said substrate and said crosslinking agent;
- and
- (ii) delivering said phase invertible composition to a site of a subject before said composition goes from said first fluid state to said second non-fluid state so that said phase invertible composition is delivered to said site of said subject in said first fluid state.
A kit for producing a fluid phase invertible composition, said kit consisting essentially of:
- (a) a fluid substrate comprising a proteinaceous material, wherein said proteinaceous material comprises one or more of:
- albumin, elastin, fibrin and soluble and insoluble forms of collagen and combinations thereof;
- (b) a heat stabilized dialdehyde crosslinking agent that has been produced by maintaining an initial dialdehyde crosslinking composition at a temperature ranging from about 45 to about 55° C. for a period of time ranging from about 1 to about 20 days;
- and
- (c) a mixing element for thoroughly combining said substrate and said crosslinker with each other to produce said fluid phase invertible composition;
- wherein said components (a) and (b) are present in separate containers in said kit.
Dependencies are inferred from the claim text.