Lysis reagent for blood cell analysis
AssigneeImmunotech
InventorsAndre van Agthoven, Jean-Pierre Daziano, John Allen Maples
Life of this patent
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Abstract
A multifunctional reagent for erythrocytes containing an amount sufficient to produce the lysis of erythrocytes or the sphering of erythrocytes in such a way that they can be detected by a cytometer or an automatic counting device, of a carbamate or of an agent inducing the formation by the erythrocytes, from carbonate and from a nitrogenated heterocycle or ammonium ions, of a carbamate combined with the absorption of CO2 by said erythrocytes, process for lysing or sphering erythrocytes and preparation process for leucocytes.
Claims28
A lysis reagent for blood cell analysis comprising;
- (a) an enzyme consisting essentially of a carbonic anhydrase in an amount sufficient to enable lysis of erythrocytes of a blood sample;
- (b) a carbonate or hydrogen carbonate salt;
- and
- (c) an ammonium salt or a heterocyclic amine or salt thereof;
- said heterocyclic amine being monocyclic with a 5 to 6 member ring;
- wherein said carbonic anhydrase has a concentration from 0.002 mg to 200 mg per liter, equivalent to from 10 to 106 Wilbur-Anderson units uer liter (W-A U/liter).
A lysis reagent for blood cell analysis comprising:
- (a) an enzyme consisting essentially of a carbonic anhydrase in a concentration from 0.002 mg to 200 mg per liter, equivalent to from 10 to 106 (W-A U/liter), to enable lysis of erythrocytes of a blood sample;
- (b) a carbonate or hydrogen carbonate salt in a concentration from 1 to 100 mM;
- and
- (c) an ammonium salt or a heterocyclic amine or salt thereof;
- in a concentration from 10 to 250 mM;
- said heterocyclic amine being monocyclic with a 5 to 6 member ring.
A lysis reagent for blood cell analysis comprising:
- (a) an enzyme consisting essentially of a carbonic anhydrase in a concentration from 0.002 mg to 200 mg per liter, equivalent to from 10 to 106 (W-A U/liter), to enable lysis of erythrocytes of a blood sample;
- (b) a carbonate or hydrogen carbonate salt in a concentration from 1 to 100 mM;
- and
- (c) ammonium chloride, morpholine chloride, pyrrolidine chloride, or piperidine chloride in a concentration from 10 to 250 mM;
- and
- (d) a buffer in a concentration from 0.5 to 30 mM.
A lysis reagent for blood cell analysis comprising:
- (a) an enzyme consisting essentially of carbonic anhydrase in an amount sufficient to enable lysis of erythrocytes of a blood sample;
- and
- (b) an ammonium salt or a heterocyclic amine or salt thereof;
- wherein said carbonic anhydrase has a concentration from 0.002 mg to 200 mg per liter, equivalent to from 10 to 106 (W-A U/liter).
Dependencies are inferred from the claim text.