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US 7,297,786 B2Granted
RNA interference in respiratory epitheial cells
AssigneeUniversity of Iowa Research Foundation
InventorsPaul B. McCray, Beverly L. Davidson, Anthony J. Fischer, Hong P. Jia, Maureen D. Donovan, Patrick L. Sinn, Mark Aaron Behlke
Life of this patent
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Abstract
The present invention is directed to small interfering RNA molecules targeted against a gene of interest in respiratory epithelial cells, and methods of using these RNA molecules.
Claims57
A precursor RNAi molecule capable of inhibiting the expression of a respiratory syncytial virus (RSV)-specific messenger RNA (mRNA) to which it corresponds comprising:
- a first oligonucleotide strand that is 22-30 nucleotides in length;
- and
- a second oligonucleotide strand that is 22-30 nucleotides in length and comprises a nucleotide sequence that is sufficiently complementary to a sequence of an RNA of a target gene to direct target-specific RNAi and wherein the second oligonucleotide strand anneals to the first oligonucleotide strand under biological conditions,
- wherein the first strand of RNA comprises SEQ ID NO:279 and the second strand of RNA comprises SEQ ID NO:280, and wherein the precursor RNAi molecule is a substrate for a Dicer.
1 more element
An isolated or purified expression cassette comprising
- a nucleic acid encoding a first oligonucleotide strand that is 22-30 nucleotides in length, wherein the first strand of RNA comprises SEQ ID NO:279;
- a second oligonucleotide strand that is 22-30 nucleotides in length, wherein the second strand of RNA comprises SEQ ID NO:280 and comprises a nucleotide sequence that is sufficiently complementary to a seciuence of an RNA of a target gene to direct target-specific RNAi;
- an oligonucleotide linker positioned between the first oligonucleotide strand and the second oligonucleotide strand;
- and
- a promoter operably linked to the first oligonucleotide strand.
2 more elements
A vector comprising two expression cassettes,
- the first expression cassette comprising a first promoter operably linked to a nucleic acid encoding a first oligonucleotide strand that is 22-30 nucleotides in length, wherein the first oligonucleotide strand comprises SEQ ID NO:279 operably linked to a promoter;
- and
- the second expression cassette comprising a second promoter operably linked to a second oligonucleotide strand that is 22-30 nucleotides in length, wherein the second oligonucleotide strand comprises SEQ ID NO:280 and comprises a nucleotide sequence that is sufficiently complementary to a sequence of an RNA of a target gene to direct target-specific RNAi.
Dependencies are inferred from the claim text.