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US 7,297,540 B2Granted
Methods of generating tissue using devitalized, acellular scaffold matrices derived from micro-organs
AssigneeYissum Research Development Company of the Hebrew University of Jerusalem
InventorEduardo N. Mitrani
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Abstract
Methods of generating, and isolating adult stem cells and utilizing such cells and/or embryonic stem cells in generating tissue of a specific function and micro-architecture are provided.
Claims10
A method of generating an artificial micro-organ comprising:
- (a) providing devitalized, acellular, tissue-derived three dimensional scaffold, said acellular three dimensional scaffold being of dimensions selected such that when populated with cells, said cells positionable deepest within said scaffold are at least about 100 micrometers and not more than about 225 micrometers away from said cells positioned at a nearest surface exposed to a source of gas and nutrients formed on said scaffold;
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- (b) seeding said acellular three dimensional scaffold with stem cells, progenitor cells or differentiated cells, wherein said differentiated cells are of the same tissue from which the scaffold was generated, and
- (c) providing conditions for cell growth and proliferation.
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Dependencies are inferred from the claim text.