Mosaic infectious bursal disease virus vaccines
AssigneeID-Lelystad, Instituut voor Dierhouderij en Diergezondheid B.V.
InventorsHendrik Johannis Boot, Anna Agnes H. M. ter Huurne, Bernardus Petrus H. Peeters
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Abstract
The invention relates to Infectious Bursal Disease Virus (“IBDV”) and vaccines therefor. Provided are infectious recombinant Infectious Bursal Disease Virus (“rIBDV”) essentially incapable of growing in a cell that is not derived from a bursa cell, or an infectious rIBDV having retained at least part of the very virulent characteristics of a very virulent Infectious Bursal Disease Virus (“vvIBDV”).
Claims11
A method for obtaining an infectious recombinant very virulent Infectious Bursal Disease Virus (vvIBDV), said method comprising:
- transfecting at least one first cell, which cell is non-permissive for vvIBDV, with a recombinant nucleic acid comprising a vvIBDV genome;
- incubating said at least one first cell in a culture medium, so as to produce recombinant vvIBDV which recombinant vvIBDV does not infect said at least one first cell, and which further retains its vvIBDV character;
- rescuing said recombinant vvIBDV from said at least one transfected first cell or said culture medium;
- and
- propagating said recovered rescued recombinant vvIBDV in at least one second cell which is permissive for said vvIBDV.
Dependencies are inferred from the claim text.