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US 7,294,479 B2Granted
Compositions, kit and one-step method for monitoring compounds having anti-factor Xa and/or anti factor IIa activities
OwnerThye Yininventor
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Abstract
Provided are compositions and methods for accurate determination of the concentration of anticoagulant in a sample such as a blood or plasma sample. The compositions can contain a Factor X compound and Factor V compound, and additional components as well. Methods for performing the assay include one-step methods in which a sample is added to a coagulation assay composition, and time is monitored from the point of adding the sample and coagulation assay composition to an endpoint, such as clot formation.
Claims20
A blood coagulation assay composition comprising components:
- a) partially or substantially pure Factor X or Factor Xa,
- b) Factor V or Factor Va,
- c) a multivalent cation,
- wherein the components of the composition are substantially unreacted with one another,
- wherein the composition is present in a single chamber vessel and
- wherein the composition is formed by, in either order,
- placing an aqueous solution comprising component a) the Factor X or
- Factor Xa and c) the multivalent cation in the vessel and
- freezing the solution,
- placing an aqueous solution comprising component b) the Factor V or
- Factor Va in the same vessel,
- freezing the solution, and
- lyophilizing the frozen solution until substantially all water is removed from the vessel, wherein the latter performed placing step is conducted substantially without allowing the previous solution to transition to liquid form.
10 more elements
Dependencies are inferred from the claim text.