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US 7,289,205 B2Granted
Fluorescence polarization imaging devices and methods
AssigneeThe General Hospital Corporation
InventorsAnna N. Yaroslavsky, Richard R. Anderson
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Abstract
The present invention is directed to a novel multi-spectral exogenous fluorescence polarization imaging technique that enables rapid imaging of large tissue fields. The imaging device includes a tunable monochromatic light source and a CCD camera. Linear polarizers are placed into both the incident and collected light pathways in order to obtain fluorescence polarization or/and anisotropy image. To acquire exogenous fluorescence image, fluorescent contrast agents are delivered to a target tissue.
Claims20
An apparatus for imaging an undyed tissue region, comprising:
- a) a polarized light emitter operable to emit excitation light having a wavelength and a first polarization direction to the undyed tissue region;
- b) a light detector operable to detect:
- i) fluorescent light remitted from the undyed tissue region having said first polarization direction;
- and
- ii) fluorescent light remitted from the undyed tissue region having a second polarization direction perpendicular to said first polarization direction;
- and
- c) an analyzer operable to form a fluorescence image based on said detected fluorescent light having said first polarization direction and said detected light having said second polarization direction, the image illustrating differing degrees to which the remitted fluorescent light has been depolarized by different undyed tissue types with respect to the excitation light.
4 more elements
A method for imaging tissue region comprising the steps of:
- a) providing an undyed tissue region to be imaged;
- b) directing excitation light having a wavelength and a first polarization direction toward the undyed tissue region;
- c) detecting fluorescence light remitted from the undyed tissue region having said first polarization direction and a second polarization direction perpendicular to said first polarization direction;
- and
- d) forming a fluorescence image based on said detected light having said first polarization direction and said second polarization direction, the fluorescence image illustrating differing degrees to which the remitted light has been depolarized by different undyed tissue types with respect to the excitation light.
2 more elements
A method for imaging a tissue region comprising the steps of:
- a) emitting excitation light having a wavelength and a first polarization direction to an undyed tissue region;
- b) detecting fluorescence light remitted from the undyed tissue region having said first polarization direction and a second polarization direction perpendicular to said first polarization direction;
- c) forming an endogenous fluorescence polarization image of the undyed tissue region based on said detected light having said first polarization direction and said second polarization direction, the endogenous image illustrating differing degrees to which the remitted light has been depolarized by different undyed tissue types with respect to the excitation light;
- d) dying said tissue region with a fluorescence contrast agent;
- e) detecting fluorescence light remitted from the dyed tissue region having said first polarization direction and a second polarization direction perpendicular to said first polarization direction;
- f) forming a total fluorescence polarization image based on said detected light having said first polarization direction and said second polarization direction;
- and
- g) forming a net fluorescence polarization of anisotropy image based on said endogenous fluorescence polarization image and said total fluorescence polarization image.
5 more elements
A method for imaging a tissue region comprising the steps of:
- a) emitting excitation light having a wavelength and a first polarization direction to an undyed tissue region;
- b) detecting fluorescence light remitted from the undyed tissue region having said first polarization direction and a second polarization direction perpendicular to said first polarization direction;
- c) forming an endogenous fluorescence anisotropy image based on said detected light having said first polarization direction and said second polarization direction, the endogenous image illustrating differing degrees to which the remitted light has been depolarized by different undyed tissue types with respect to the excitation light;
- d) dying said tissue region with a fluorescence contrast agent;
- e) detecting fluorescence light remitted from the dyed tissue region having said first polarization direction and a second polarization direction perpendicular to said first polarization direction;
- f) forming a total fluorescence anisotropy image based on said detected light having said first polarization direction and said second polarization direction;
- and
- g) forming a net fluorescence anisotropy image based on said endogenous fluorescence anisatropy image and said total fluorescence anisotropy image.
5 more elements
Dependencies are inferred from the claim text.