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US 7,285,420 B2Granted
System and method for self-referencing a sensor in a micron-sized deep flow chamber
AssigneeCorning Incorporated
InventorsNorman H. Fontaine, Prantik Mazumder, Mark A. Quesada, Eric J. Mozdy, Po Ki Yuen
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Abstract
A system and method are described herein for self-referencing a sensor that is used to detect a biomolecular binding event and/or kinetics which occur in a sample solution flowing along side a reference solution in a micron-sized deep flow channel.
Claims17
A method for self-referencing a sensor associated with a micron-sized deep flow channel, said method comprising the steps of:
- directing an optical beam at said sensor which has a single sensing region divided into a detection region and a reference region which are contiguous to one another and which are respectively interfaced with a sample solution and a reference solution that flow side-by-side to one another in a longitudinal direction within said micron-sized deep flow channel;
- receiving an output optical beam from said sensor;
- analyzing the output optical beam to determine a detection signal associated with the sample solution flowing in the detection region of said sensor and to determine a reference signal associated with the reference solution flowing in the reference region of said sensor;
- and
- subtracting the reference signal from the detection signal to generate a corrected detection signal, wherein uncertainties in the detection signal due to environmental conditions and noise generated by components in an interrogation system are reduced in the corrected detection signal.
2 more elements
An interrogation system capable of performing the following steps:
- directing an optical beam at a sensor which has a single sensing region divided into a detection region and a reference region which are contiguous to one another and which are respectively interfaced with a sample solution and a reference solution that flow side-by-side to one another in a longitudinal direction within a micron-sized deep flow channel;
- receiving an output optical beam from said sensor;
- wherein the output optical beam is analyzed to determine a detection signal associated with the sample solution flowing in the detection region of said sensor and to determine a reference signal associated with the reference solution flowing in the reference region of said sensor;
- and
- wherein the reference signal is subtracted from the detection signal to generate a corrected detection signal;
- and
- wherein the corrected detection signal indicates whether or not a biomolecular binding event occurred in the sample solution flowing in said micron-sized deep flow channel.
4 more elements
Dependencies are inferred from the claim text.