Genes of cell wall-degrading enzymes derived from aspergillus, and method for the production the enzymes

An isolated polynucleotide sequence comprising one of the following DNAs:

• (a) a DNA consisting of a nucleotide sequence of SEQ ID No:
• 1,
• (b) a DNA which hybridizes with a nucleic acid comprising the nucleotide sequence of SEQ ID No:1 or its complete complement thereof under stringent conditions comprising a sodium concentration of 150 mM at a pH of 6-8 and 68° C. and encoding a protein having exo-β-1,3-glucanase activity.

An isolated polynucleotide sequence encoding one of the following proteins:

• (a) β-1,3-glucanase obtained from Aspergillus oryzae and having a molecular weight of 80 kD, and
• (b) a protein consisting of an amino acid sequence of SEQ ID No:4.

An isolated polynucleotide sequence comprising one of the following DNAs:

• (a) a DNA consisting of a nucleotide sequence of SEQ ID No:3, and
• (b) a DNA, which hybridizes with a nucleic acid comprising the nucleotide sequence of SEQ ID No:3 or its complete complement thereof under stringent conditions comprising a sodium concentration of 150 mM at a pH of 6-8 and 68° C. and encoding a protein having endo-β-1,3-glucanase activity.