RNA interference pathway genes as tools for targeted genetic interference
AssigneesUniversity of Massachusetts, Carnegie Institute of Washington
InventorsCraig C. Mello, Andrew Fire, Hiroaki Tabara, Alla Grishok
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Abstract
Genes involved in double-stranded RNA interference (RNAi pathway genes) are identified and used to investigate the RNAi pathway. The genes and their products are also useful for modulating RNAi pathway activity.
Claims7
A substantially pure polypeptide encoded by a nucleic acid molecule which hybridizes under high stringency conditions of hybridization at 68° C. in 5×SSC/5× Denhardt solution/1.0% SDS, followed by washing in 0.1×SSC/0.1% SDS at 68° C. to a complement of the nucleic acid molecule set forth as SEQ ID NO:2, wherein the polypeptide mediates RNA interference (RNAi).
A substantially pure polypeptide fragment comprising amino acids 203 to 1020 of SEQ ID NO:3.
A substantially pure protein encoded by the nucleic acid molecule set forth as SEQ ID NO:2.
A substantially pure protein comprising the amino acid sequence of SEQ ID NO:3.
A fusion protein comprising the polypeptide of any one of the preceding claims and a heterologous polypeptide.
Dependencies are inferred from the claim text.